TA Cloning: Simple & Easy Cloning Method
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2019-10-13に共有
It is an easy and simple method of cloning compared to the traditional sub cloning.
TA cloning is based on the Adenine Thymine base pairing on different DNA fragments to hybridize.
PCR product is generated using Taq Polymerase, which adds A overhangs to the 3’end of the product.
PCR product with the A overhang can be ligated to linearized vector having T overhang