in vitro Transcription

Henrik's Lab
Henrik's Lab
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Published 2023-05-29
With in vitro transcription it is possible to produce an RNA molecule from a DNA template in a simple reaction tube. There are different kits and protocols on the market. How in vitro transcription works and why the molecular laboratory technique is such a powerful tool for biologists will be explained in this video.



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0:00 Intro
0:38 Protocol
0:50 1. DNA template generation
1:51 2. Transcription reaction
2:41 3. Purification
3:10 What can be done with in vitro Transcription?
3:37 Outro



#invitro #transcription #molecularbiology
All Comments (5)
  • @03500
    Amazing thank you so much 😊
  • @wichetleelamanit6195
    Thanks for the video. I guess the technique now is not complicated. If you add more advanced details, it will be more useful.
  • @richardc6998
    Awesome video, I have a question tho: If I want to increase gene expression what do I have to do? Add more polymerases? Thank you!
  • @alineferrero2152
    aweesome video! do you have any paper that I can read the protocol? thanks!