MIC (Broth Microdilution) Testing

TheRubinLab
TheRubinLab
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Published 2020-07-27
In this video you’ll learn the principle of the broth-microdilution susceptibility test and see how it is performed and interpreted.

Useful links:
CLSI veterinary interpretive criteria: clsivet.org/GetDoc.aspx?doc=CLSI%20VET08%20ED4:201…
European Committee on Antimicrobial Susceptibility Testing (EUCAST): eucast.org/

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All Comments (12)
  • @nealg7029
    Hi, do you have a link where you got this protocol from CLSI? I can't seem to find the broth micro-dilution method protocol to follow :/ It would be helpful for my thesis! Thanks in advance for, great content!
  • @Tobias-uo7vo
    Thx for the nice Video. Do you have experience with Broth Micro Dilution for Helicobacter pylori?
  • @victorkofoed3258
    Thanks, very informative. I was just wondering, is it recommended to wear a mask for protection while performing this method, like in the video, or was that in relation to covid?
  • @braulioteixeira116
    Why should we use double strength medium for microbroth dilution test? Should we use double strength concentrations for the antimicrobials (controls) and the tested products too?
  • @himensalimizand5074
    Sometimes for certain antibiotics instead of complet growth of bacteria in the well, there is pin point growth. Should it be considered as resistant or ignore it?
  • @judithgracia5785
    Can I know, What volume of inoculated culture do you add to each well? Thank you
  • @55prasenjit
    At 4 min, where did you get the picture from? Can i use it for my project?
  • @prabinjirel591
    in which wavelength microplate is read? Incase of colistin broth microdilution.